Application

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected PXR in 10 µg of human liver tissue lysate.

Detect PXR using this Anti-PXR Antibody, clone 1F10.1 validated for use in Western Blotting.

General description

Nuclear receptor subfamily 1 group I member 2 (UniProt O75469; also known as Orphan nuclear receptor PAR1, Orphan nuclear receptor PXR, Pregnane X nuclear receptor, Steroid and xenobiotic receptor, SXR) is encoded by the NR1I2 (also known as BXR, PAR, PRR, PXR, SAR, SXR, ONR1) gene (Gene ID 8856) in human. Pregnane X receptor (PXR) is a nuclear receptor that displays similarity to PPAR and RXR. PXR is activated by a large number of endogenous and exogenous chemicals including steroids and antibiotics. One of the key roles of PXR is the induction of CYP3A4, a P450 enzyme involved in drug metabolism.

Immunogen

KLH-conjugated linear peptide corresponding to human PXR.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in human colon tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected PXR in 10 µg of human colon tissue lysate.

Specificity

Epitope is present in all 7 alternative spliced isoforms (isoform 1A/PRR1-A, 1B/PRR1-B, 1C/PRR1-C, 2A/PRR2-A, 2B/PRR2-V, 2C/PRR2-C, and isoform 3).

Target description

~53 kDa observed. Uniprot describes 7 isoforms produced by alternative splicing ranging from ~40 kDa-54 kDa